Subtyping of Streptococcus uberis by DNA amplification fingerprinting

Subtyping of Streptococcus uberis by DNA amplification fingerprinting.

Total DNA of Streptococcus uberis from cows with mastitis was analyzed by DNA amplification fingerprinting (DAF) and compared with restriction endonuclease fingerprinting (REF). DAF grouped 22 strains into 15 distinct patterns, while REF grouped them into 12 patterns. These results suggest that DAF is a useful technique for subtyping strains of S. uberis.

Detection of new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections by polymerase chain reaction-based DNA fingerprinting.

Polymerase chain reaction-based DNA fingerprinting was used as a tool to differentiate new and persistent Streptococcus uberis and Streptococcus dysgalactiae intramammary infections (IMI) in dairy cows. The same subtype of S. uberis or S. dysgalactiae was detected from some infected mammary glands from one lactation to the next documenting the persistence of these infections. Conversely, some s...

DNA fingerprinting by infrequent-restriction-site amplification.

Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce strain-specific electrophoretic patterns from c...

Activation of bovine plasminogen by Streptococcus uberis.

Culture filtrate from Streptococcus uberis was found to activate bovine and equine plasminogen but not that from rabbit, human or porcine plasma. In contrast, streptokinase from a Lancefield group C Streptococcus activated human plasminogen but not that from bovine, porcine and rabbit plasma. Very slight activity was observed against equine plasminogen. Plasmin was detected by hydrolysis of ski...

DNA Fingerprinting by PCR Amplification of HLA Genes